Composite

Part:BBa_K4115046:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 15:14, 1 October 2022 by Yurj (Talk | contribs)


NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2145


Design Notes

We use sfGFP as a reporter gene to detect whether LuxR is functional when responding to the AHL signal in HL7942. It is the first step in our demonstration that Escherichia coli and Synechococcus can communicate through quorum sensing signals.


Source

The sequence of LuxR is achieved from the article 'Tools for engineering coordinated system behaviour in synthetic microbial consortia'[1]. The sequence of sfGFP(BBa_K4115000) is designed by our team. Most components in the composite part are synthesized by the company and then we integrate them into the backbone of PUC57.

References

[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2