Composite
Part:BBa_K4268017
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-09-30)
Revision as of 00:20, 1 October 2022 by Jazmine124 (Talk | contribs)
Untagged Capsid and Core Multi-Transcriptional Unit
Usage and Biology
This is a Golden Gate Assembly Level 2 part. This part joins transcriptional units 1, 2, 4, and 5 – this includes head-tail connector, capsid assembly protein, capsid protein with tag, and tail tublar protein A
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3806
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3806
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5110
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3806
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3806
Illegal NgoMIV site found at 3446
Illegal NgoMIV site found at 3585
Illegal NgoMIV site found at 4541 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |