Composite

Part:BBa_K4268016

Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski   Group: iGEM22_SUNY_Oneonta   (2022-09-30)
Revision as of 00:16, 1 October 2022 by Jazmine124 (Talk | contribs)

Neck and Tail Multi-Transcriptional Unit


Usage and Biology

This is a Golden Gate Assembly Level 2 part. This part joins transcriptional units 1, 2, 3, and 5 – this includes head-tail connector, capsid assembly protein, capsid protein with no tag, and tail tublar protein A respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 398
    Illegal NheI site found at 4934
    Illegal PstI site found at 1760
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1760
    Illegal NgoMIV site found at 6431
    Illegal AgeI site found at 8462
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None