Part:BBa_K4268008
Head-Tail Connector Transcriptional Unit
Usage and Biology
S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle (Shitrit et al., 2021). T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host (Raytcheva et al., 2011).
This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.
However, it will infect Synechococcus sp WH 8109, the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as Synechococcus sp PCC 11901.
The Head-Tail Connector Transcriptional Unit classifies as a Level 1 Transcriptional Unit of Golden Gate Assembly. A strong promoter and RBS were used, BBa_R0085 + BBa_B0034. The terminator used was BBa_B0015.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 395
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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