Regulatory

Part:BBa_K4452000:Design

Designed by: iGEM22_Hopkins   Group: iGEM22_Hopkins   (2022-09-30)
Revision as of 05:13, 9 October 2022 by KalenClifton (Talk | contribs) (replace source with correct article)

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PLT2 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the PLT2 promoter, there is a long, 5.8kb version and a shorter 1.3 kb version. We have decided to use the short (1.3 kb) PLT-2 promoter because its shorter length makes it easier to assemble.


Source

Galinha, C., Hofhuis, H., Luijten, M. et al. “PLETHORA proteins as dose-dependent master regulators of Arabidopsis root development”. Nature 449, 1053–1057 (2007). https://doi.org/10.1038/nature06206

References