Part:BBa_K200007

Cellulase

This is a BioBrick for cellulase production in E.coli. This biobrick codes for CelE cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.1

Cellulase mainly catalyses the reactions that changes crystalline cellulose to cellobiose2 and then finally to glucose. It also catalyses, to a small extent, the break down of carboxymethyl cellulose. This cellulase is protease resistant.

As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine. It will then digest the cellulose found in our food.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 700
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 287
  Illegal BamHI site found at 814
  Illegal XhoI site found at 524
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 199
  Illegal PstI site found at 700
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

1. 1 pmid=9055408
2. 2 pmid=10714996

</biblio>