![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K4212049:Design
Designed by: Yuancheng Ding Group: iGEM22_Imperial_College_London (2022-09-30)
Ger1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3591
Illegal BglII site found at 3123
Illegal XhoI site found at 713 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542
Illegal NgoMIV site found at 66 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2778
Design Notes
PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter.
Source
Synthetic construct.