Coding

Part:BBa_K200007

Designed by: Tianyi Wang   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 10:06, 28 August 2009 by Dineka (Talk | contribs)

Cellulase

This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.

Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.


As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 199
    Illegal PstI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI]

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