Coding

Part:BBa_K4117888

Designed by: He Du,Yue Zhang   Group: iGEM22_BNU-China   (2022-09-30)
Revision as of 18:27, 11 October 2022 by Qiyueyao (Talk | contribs)


UGT1A3

UGT1A3 in this pathway plays a significant role in metabolic section, working upon the sensing section. Tetrahydrocannabinol (Δ9 -THC), the primary psychoactive ingredient in marijuana, is subject to cytochrome P450 oxidation and subsequent UDP-glucuronosyltransferase(UGT)-dependent glucuronidation. In our system, Δ-9-ThC is hydroxylated by CYP2C9 (BBa_K4117002) and CYP2C19 (BBa_K4117022), remaining psychoactive. The major product of CYP2C9 and CYP2C19 metabolism, 11-OH-Δ-9-ThC, was further oxidized to THC-COOH. Then UGT1A3, which catalyzes the glucuronidation of the former substrate, increases the metabolite's water solubility, thereby facilitating excretion into the urine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4599
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4599
    Illegal BglII site found at 4257
    Illegal BamHI site found at 2887
    Illegal BamHI site found at 3444
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal AgeI site found at 3031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732


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Categories
Parameters
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