Coding
Part:BBa_K4242000
Designed by: zhelin Jiang Group: iGEM22_CUG-China (2022-07-12)
Revision as of 14:56, 30 September 2022 by Jiangzhelin (Talk | contribs)
FleQ
coding region for FleQ protein, which is a transcription factor that can be combine with FleN and c-di-GMP to regulate the gene transcription under the promoter Ppel.
Usage and Biology
FleQ can control flagella biosynthesis and exopolysaccharide (EPS). scientists found FleQ can activate the expression of flagella biosynthesis genes but inhibit the expression of exopolysaccharide genes. It has an important Usage we can regulate FleQ to control biofilm information and explore medical research.
FleQ protein bend with DNA
* Lewis et al. Proceedings of the National Academy of Sciences, 2011.Sequence and Features
Blast result of our site-removed FleQ
References
[1]Matsuyama BY, Krasteva PV, Baraquet C, Harwood CS, Sondermann H, Navarro MV. Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):E209-18. [2]Hickman JW, Harwood CS. Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor. Mol Microbiol. 2008 Jul;69(2):376-89. [3]Baraquet C, Murakami K, Parsek MR, Harwood CS. The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP. Nucleic Acids Res. 2012 Aug;40(15):7207-18.
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Categories
Parameters
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