Coding
Part:BBa_K4425002
Designed by: Junfei Xia Group: iGEM22_SUSTech_Shenzhen (2022-09-30)
PtUGT
a glucosyltransferase that can introduce a reversible protecting glucose group into indoxyl, turning low water solubility product indigo (or tyrian purple) to soluble indican.
Usage and Biology
gene of PtUGT and FMO was assembled with pET28a backbone to construct pET28a-PtUGT-FMO. PtUGT and FMO were co-expression to convert indole (or 6-Br-indole) to indican which gives a sustainable indigo dyeing strategy.
Source
PtUGT come from P. tinctorium
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 886
Illegal AgeI site found at 1093 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 484
Reference
Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.
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Categories
Parameters
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