Composite

Part:BBa_K4345018

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 10:56, 5 October 2022 by Pventuba (Talk | contribs)


Tet/lac promoter - ccdB fused to sfGFP

Design of the thermoregulated translation of toxin ccdB. Fluorescent protein sfGFP was linked to the antitoxin for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//cds/transcriptionalregulator/activator
Parameters
control
emission
excitation