Regulatory
Part:BBa_K4468001
Designed by: Zhichao Li Group: iGEM22_HUST-China (2022-09-30)
Revision as of 09:08, 30 September 2022 by Zhichao Li (Talk | contribs)
PgolB
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1802
Illegal AgeI site found at 50
Illegal AgeI site found at 399
Illegal AgeI site found at 1337 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The GolS of Salmonella is a transcriptional regulator comes from the gold specific family MerR. Its expressed protein GolS plays a role as an operon that responsible for regulating the promoter PgolB. Upon recognizing and adsorbing extracellular Au3+, the expression of downstream genes after promoter PgolB will be activated. PgolB is the promoter of the GolS system. The expression of genes after PgolB is strictly regulated by GolS. In the absence of Au3+, GolS inhibits PgolB to prevent downstream genes’ expression. Whereas when GolS adsorbs exogenous Au3+, the inhibition turns to cease, allowing PgolB to initiate expression.
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Categories
Parameters
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