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Coding
Part:BBa_K4307003
Designed by: Chi Zhang Group: iGEM22_Tsinghua (2022-09-29)
Bxb1 integrase
There are two operators in the genome of bacteriophage, called OR (the right operator) and OL (the left operator) respectively. OR plays an important role in the regulation of gene expression of phage. OR composed of three parts, called OR1, OR2 and OR3. The isolated OR1-OR2 can function as promoter and can be inhibit by cI protein, a repressor expressed by bacteriophage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 180
Illegal BamHI site found at 558 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 973
Illegal AgeI site found at 1333 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |