Coding

Part:BBa_K4298006:Design

Designed by: Buyao Li   Group: iGEM22_Peking   (2022-09-29)
Revision as of 12:09, 12 October 2022 by GrizzlyZhao (Talk | contribs)

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TM8.3 with α-factor secretion signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 882
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Design Notes

Together TM8.3 with α-secretion signal that TM8.3 can be secreted out of yeast cell.

Source

Directed synthesis.