Coding

Part:BBa_K4447004

Designed by: Rafael Garcia Gomez   Group: iGEM22_TecMonterrey_GDL   (2022-09-29)
Revision as of 19:32, 29 September 2022 by Rafa1470 (Talk | contribs)


FRET-based system for the detection of erythromycin

FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase (BBa_K4447001),an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP (BBa_K1159302)as an energy donor and Venus (BBa_K1907000)as an energy acceptor.


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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2562



=Selecting Fluorescent Proteins=
[edit]
Categories
Parameters
None