Coding
VHH

Part:BBa_K4387995:Design

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-29)
Revision as of 19:11, 29 September 2022 by Nathi (Talk | contribs) (→‎References)


Monovalent Anti-Tumour Necrosis Factor Nanobody (VHH#3E)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The humanized amino acid sequence was converted into DNA sequence using ... and then codon optimized for E. coli with the help of the codon optimization tool provided by Integrated DNA Technologies (IDT).


Source

The amino acid sequence was directly taken from the patent mentioned in the description. [3]

References

  • [1] Harmsen, M.M., De Haard, H.J. Properties, production, and applications of camelid single-domain antibody fragments. Appl Microbiol Biotechnol 77, 13–22 (2007). https://doi.org/10.1007/s00253-007-1142-2
  • [2] Zimmermann, I., Egloff, P., Hutter, C.A.J. et al. Generation of synthetic nanobodies against delicate proteins. Nat Protoc 15, 1707–1741 (2020). https://doi.org/10.1038/s41596-020-0304-x
  • [3] Silence, Karen, Lauwereys, Marc, De Haard, Hans, et al. "Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor", Int. Publication Number: WO 2004/041862 A2, 21 May 2004