Coding

Part:BBa_K4229036

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)
Revision as of 18:11, 10 October 2022 by NikitaEdel (Talk | contribs)


T3 protein from the full wiffleball of the BMC

The T3 proteins builds together with the T2-protein(BBa_K4229035) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049).

A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein.
























The plasmid with this protein was sent kindly send to us by the Kerfeld Group after we found this very interesting paper:

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 566
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 186
    Illegal NgoMIV site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None