Part:BBa_K4192010
cdg gene from Pseudomonas fluorescens str.2P24
The cdg (BBa_K4192010) encodes the DGC protein of Pseudomonas fluorescens,also named as dgc. DGC protein catalyzes the synthesis of r c-di-GMP,which is an important regulator[1]. It can promote bacterial adhesion, extracellular polysaccharide synthesis and other functions. Increasing its expression can increase the production of biofilm.
Characterization
We increased the expression number of cdg gene in Pseudomonas fluorescens 2P24 and tested its role in increasing biofilm production
We connected it to the downstream of Plac to obtain the part BBa_K4192113, and used crystal violet staining to quantitatively test its role in biofilm production. To get more information, please see https://parts.igem.org/Part:BBa_K4192114.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 263
Illegal BamHI site found at 254
Illegal XhoI site found at 352
Illegal XhoI site found at 796 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 187
Illegal AgeI site found at 754 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 205
Illegal BsaI.rc site found at 772
References
[1]Ivanov, Ivan E et al. “Atomic force and super-resolution microscopy support a role for LapA as a cell-surface biofilm adhesin of Pseudomonas fluorescens.” Research in microbiology vol. 163,9-10 (2012): 685-91. doi:10.1016/j.resmic.2012.10.001
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