Reporter

Part:BBa_K4242011:Design

Designed by: zongheng Wei   Group: iGEM22_CUG-China   (2022-09-29)
Revision as of 02:10, 9 October 2022 by Jiangzhelin (Talk | contribs) (→‎Design Notes)


Ppel+RBS+gfp+TT(pHYD-1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 817


Design Notes

this is a composite part that uses GFP to find out whether tandem promotor PcI/pel works in E. coli and S. oneidensis.r working in the plasmid.

Source

Use RBS to produce the GFP protein to reflect Ppel whether working.

References