Composite

Part:BBa_K4256188:Design

Designed by: Jingke Wang   Group: iGEM22_UCAS-China   (2022-09-29)
Revision as of 17:41, 11 October 2022 by SunJi UCAS (Talk | contribs) (→‎Source)

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EforRed validation system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 651
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 651
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The use of eforRed for chromoprotein is a part borrowed from other iGEM teams; BglII is added before and HindIII is added afterwards to facilitate subsequent vector linkage and enzyme identification for recovery


Source

It comes from the combination of composite parts BBa_I712074, BBa_K3716001, BBa_K4256133, BBa_B0012, BBa_K4256100 and BBa_K592012, and the source of each part can be checked.

References