Regulatory

Part:BBa_K4140017

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 07:47, 29 September 2022 by Ahmed Wael (Talk | contribs) (→‎Part Description)


CMV promoter

Part Description

CMV (cytomegalovirus) promoter is a strong constitutive promoter commonly used in mammalian expression systems to produce high levels of expression, However CMV promoter is on of the most variable types of promoter as it is highly variable between different cell types such as being strong in some cell types and being much weaker in other types

Usage

CMV (cytomegalovirus) promoter is a potent promoter commonly used in mammalian expression systems we use it to regulate the expression of Cas12g which have a markable role on tuning and controlling the levels of PAH within normal levels.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None