Part:BBa_K4193000
RNA aptamer 1
RNA that can combine with GABA
Design
We first used the secondary structure of the aptamer and the specific binding with the ligand to de novo design the aptamer, and carried out molecular docking and dynamic simulation for testing. Then we got aptamer GABA-1.
Characterization
Our team simulated RNA aptamer with high specificity for γ- aminobutyric acid(GABA) by modeling method. We then synthesized the target RNA aptamer sequence with fluorescence 3’ FAM. Therefore, the dissociation constant(Kd) of the aptamer was determined by fluorescence spectroscopy. We incubated the aptamer with a range of concentrations of GABA for two hours at room temperature, respectively. Next, we measured the fluorescence intensity at 525nm of each group and used the following formula to characterize the Kd value:
Result
After statistical analysis, we found that GABA-1 had certain capacity of transporting, but it didn’t work well as we expected. That’s why we built GABA-2.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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