Part:BBa_K4140022

Peg10

This is a paternally expressed imprinted gene that binds its own mRNA and self-assembles into virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA. It is thought to have originated from the Ty3/Gypsy family of retrotransposons

Usage

PEG10 mRNA can be transferred between cells thanks to a paternally expressed imprinted gene that binds its own mRNA and self-assembles into extracellular vesicles that resemble virion capsules. Making VLPs is one of the SEND platform's components for mRNA delivery. Therefore, we took advantage of it by attaching the 5' and 3'UTR of PEG10 to the mRNA of PAH in order to make the PEG10 protein encapsulate the PAH mRNA.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1117
  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 240
  Illegal BamHI site found at 477
  Illegal BamHI site found at 1160
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1045
  Illegal PstI site found at 1747
  Illegal AgeI site found at 376
  
• 1000
  COMPATIBLE WITH RFC[1000]