Part:BBa_K4140021
AcrIIA5 v2 anti-CRISPR
Part Description
Anti CRISPR for Cas12g Anti CRISPR are small proteins (approximately, 12–193 amino acids) which have become, quickly a new method to regulate CRISPR system activity by altering the nuclease activity or hindering binding to the target gene
Usage
We have thought to work on developing powerful protein designs to enable us to control the specificity and limit off-targeting of cas proteins. We have been relying on our protein evolution models that use deep learning to develop and predict new mutants of anti crispr proteins that are cas-specific and can act as an on-demand safety component for cas-based biosynthetic designs. This biosafety aspect is integrated with our therapeutic project for this year to develop our sensitive therapeutic design with natural brake elements (Acr-proteins) deployed to assure the safety of our design.
Literature Characterization
References
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 31
Illegal EcoRI site found at 521
Illegal XbaI site found at 25
Illegal XbaI site found at 530
Illegal SpeI site found at 19
Illegal SpeI site found at 539
Illegal PstI site found at 1
Illegal PstI site found at 565 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 31
Illegal EcoRI site found at 521
Illegal SpeI site found at 19
Illegal SpeI site found at 539
Illegal PstI site found at 1
Illegal PstI site found at 565
Illegal NotI site found at 43
Illegal NotI site found at 502 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 31
Illegal EcoRI site found at 521
Illegal BglII site found at 13
Illegal BglII site found at 548
Illegal BamHI site found at 37
Illegal BamHI site found at 513 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 31
Illegal EcoRI site found at 521
Illegal XbaI site found at 25
Illegal XbaI site found at 530
Illegal SpeI site found at 19
Illegal SpeI site found at 539
Illegal PstI site found at 1
Illegal PstI site found at 565 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 31
Illegal EcoRI site found at 521
Illegal XbaI site found at 25
Illegal XbaI site found at 530
Illegal SpeI site found at 19
Illegal SpeI site found at 539
Illegal PstI site found at 1
Illegal PstI site found at 565 - 1000COMPATIBLE WITH RFC[1000]
None |