Part:BBa_K4140004
P2A linker
Part Description
In order to mediate the "cleavage" of two proteins, 2A peptide sequences were discovered in Picornaviruses. Effective multiple protein expression from a single open reading frame (ORF) is achieved using 2A peptide-linked multicistronic vectors. Use alternative 2A peptide sequences when linking more than two genes to reduce the likelihood of homologous recombination. All eukaryotic systems studied to date, including mammalian cells, yeast, and plants, have effectively utilised the 2A peptide system.
Usage
Before implementing our therapeutic circuit product into a human body, we employed this 2A linker to attach a reporter gene to it so that we could test its functionality and ability to express PAH by utilizing spectrophotometry to monitor the degree of PAH expression.
Literature Characterization
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |