RBS

Part:BBa_K4140002

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 17:38, 28 September 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


TyrR RBS


Part Description

Sequence upstream of the start codon is crucial for the recruitment of a ribosome during the initiation of translation, which influences the rate and efficiency at which a certain Open Reading Frame (ORF) is translated by the following mechanisms:
-the rate of ribosomal recruitment to RBS
-the speed at which a recruited ribosome is able to start translation (i.e. the translation initiation efficiency)
-The ideal spacing of the RBS from the start codon

Usage

We use this part to increase the expression of TyrR by increasing the rate of ribosomal recruitment and the ability to start and influences the precision and effectiveness with which the mRNA translation process codon.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None