Composite

Part:BBa_K4509569:Design

Designed by: MARIE JYOTSNA JAWAHAR, Vibha Murali, Rhea Harry William   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 18:45, 28 September 2022 by Marie JJ (Talk | contribs)


HORSERADISH PEROXIDASE with constitutive promoter J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter J23119 is replaced with J23100. This switch in the design will reduce the burden in the cell during over expression of enzymes.


Source

To be added

References