DNA
Making Bio
Part:BBa_K4329001
Designed by: Yuxin He Group: iGEM22_BS_United_China (2022-09-24)
Overexpression of dethiobiotin synthase and biotin synthase in E. coli
What is it?
In order to alter the metabolic pathway of E. coli and tend to a more productive way of Biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of desthiobiotin synthase and biotin synthase. This plasmid is regulated by the T7 promoter, Lac operator, and T7 terminator.
Usage and Biology
With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/basic_part
//cds/biosynthesis
//cds/enzyme
//cds/biosynthesis
//cds/enzyme
function |