Composite
Part:BBa_K4509769:Design
Designed by: MARIE JYOTSNA JAWAHAR, Rhea Harry William, Vibha Murali Group: iGEM22_REC-CHENNAI (2022-09-28)
Cell surface aphA with constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 966
Design Notes
The constitutive promoter BBa_J23100 has been used since the strength of BBa_J23100 is more. This will facilitate overexpression of the protein on the cell surface.