Regulatory

Part:BBa_K4140006

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 17:44, 28 September 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


TyrP promoter


Part Description

The Escherichia coli K-12 chromosome has the tyrP promoter, which codes for a part of the tyrosine-specific transport system, at min 42. When phenylalanine and the tyrR gene product are together, tyrP transcription is promoted

Usage

TyrP promoter,normally regulate the expression for a part which codes for tyrosine-specific transport system, it is induced when phenylalanine and the tyrR gene product (TyrR dimer)are together ,taking advantage of that we use it to regulate the expression of PAH and ß-galactosidase.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None