Protein_Domain

Part:BBa_K157012:Design

Designed by: Kristian Müller   Group: iGEM08_Freiburg   (2008-10-26)
Revision as of 21:15, 21 October 2009 by Kristian (Talk | contribs) (→‎Design Notes)

Strep affinity tag II; Freiburg standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
Attention this part contains a double NgoMIV restriction site.

Source

Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.

References

Not a comprehensive list

<biblio>

  1. Schmidt93 pmid=8433964

// this looks to be the original reference for the Strep-tag

  1. Skerra96 pmid=8636976

// original reference for Strep-tag II

  1. Voss97 pmid=9415448

// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.

  1. Skerra99 pmid=10796988
  2. Skerra00 pmid=11036648

// history of Strep-tag system

  1. Skerra07 pmid=17571060

// includes brief overview of Strep-tag system </biblio>