Coding
NarX H399-

Part:BBa_K4345008

Designed by: Michelle Patricia   Group: iGEM22_KU_Leuven   (2022-09-28)
Revision as of 16:08, 7 October 2022 by Michadiutama (Talk | contribs)


NarX H399-E

The mutant was designed based on a study of Cavicchioli et al., (1995). The histidine on place 399 was replaced by glutamic acid. This allows the NarX mutants to dimerize but blocks it from phosphorylating NarL (the second messenger).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 659
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 659
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 659
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/phosphorylation
//cds/membrane/receptor
Parameters
activation_coeficient
protein
signalling_molecule