Coding

Part:BBa_K4228000

Designed by: Keyao Zhao   Group: iGEM22_Tongji_China   (2022-09-27)
Revision as of 16:59, 11 October 2022 by Fork (Talk | contribs)


Bromelain from pineapple

Bromelain is a mixture of enzymes derived from pineapple. Its effects are mainly a product of its proteolytic activity, which stimulates fibrinolysis by increasing plasmin.

Brief introduction

Bromelain is a group of thiol hydrolytic proteases extracted from the tropical plant pineapple and mainly exists in the fruit, bud, leaf and stem of pineapple, with a molecular weight of 33000. It belongs to the papain family of cysteine proteases, and the enzymatic activity is dependent on the thiol group of a cysteine residue within its active site. Bromelain has a variety of properties, including anti-cancer activity, anti-inflammatory effect, antimicrobial effect, antibiotic potentiation, skin protection, postsurgery recovery and so on. Therefore, it has a wide range of applications in the medical and food fields. We choose fodder processing as the application scenario of the study. Bromelain is a plant protease, which can convert protein in feed into peptides and small peptides easily absorbed by animals, improve the conversion rate of feed, so as to reduce the pollution of breeding industry to the environment. It also has a certain therapeutic effect on diarrhea caused by pathogenic bacteria, avoiding the negative effects of antibiotics, improving the growth performance of animals, and resisting the damage of parasites to animals.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 312
    Illegal BamHI site found at 1
    Illegal XhoI site found at 646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


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