Composite
XDH

Part:BBa_K4324001:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-09-27)
Revision as of 01:28, 28 September 2022 by PenTest-duck (Talk | contribs) (→‎Source)


lacPO-RBS-XDH-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 357


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.



Source

LacPO: 

- RBS: 

- CDS: XYL2 gene from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Taken from the Uniprot database.

- Terminator:

References