DNA
VR8 bindin

Part:BBa_K176042:Design

Designed by: Jiayi Dou, Hao Jiang   Group: iGEM09_USTC   (2009-08-13)
Revision as of 07:49, 13 August 2009 by Horigen (Talk | contribs) (→‎Source)

Reverse primer VR8 (K176052) binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.


Source

Reverse complement of VR8 (K176052). Picked from random sequences.

Main parameters for picking VR3~VR12:

Size: 20 bp (N19 + G or C)

Tm: VF2 Tm ±1℃

GC%: 50%

Max Self Complementarity: 3 bp

Max 3' Self Complementarity: 2 bp

Max Pair Complementarity: 3 bp

Max 3' Pair Complementarity: 2 bp

Max 3' Stability: 3.36 kcal/mol

Max Template(pSB1A3) Mispriming: 6 bp

Max Mispriming With Other VR3~VR12 Sites: 4 bp

References