Coding
Part:BBa_K4220008
Designed by: Xiaoya Liu Group: iGEM22_NEU_CHINA (2022-09-25)
P2Y2-P2A-mCherry
Introduction
The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.
P2Y2
In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
P2A
After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
mCherry
To report the successful cleavage of P2A peptide.
Results
1.The P2A peptide can work effectively:
2.The production of P2Y2:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1095
Illegal AgeI site found at 389 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 570
Illegal SapI site found at 615
[edit]
Categories
Parameters
None |