Coding

Part:BBa_K4158012

Designed by: Satohiro Takizawa   Group: iGEM22_Waseda_Tokyo   (2022-09-23)
Revision as of 07:28, 25 September 2022 by S Okuda (Talk | contribs)


SRTF1(E coli Codon Optimized sequence)

RBS and coding site for the SRTF1　protein with TEV site and His tag. SRTF1 binds to SRTF1 regulator and works as repressor. Progesterone((1S,3aS,3bS,9aR,9bS,11aS)-1-Acetyl-9a,11a-dimethyl-1,2,3,3a,3b,4,5,8,9,9a,9b,10,11,11a-tetradecahydro-7H-cyclopenta[a]phenanthren-7-one) binds to SRTF1 and inhibit its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal BamHI site found at 532
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
    Illegal AgeI site found at 173
    Illegal AgeI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None