Coding

Part:BBa_K4194007

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)
Revision as of 02:26, 29 September 2022 by HJ (Talk | contribs)

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EGFP（+LVA）

a strong reporter. EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Ex:488nm Em :507nm EC :55,900(M-1 cm-1) QY:0.6 Brightness:33.54 pKa:6.0 Maturation:25.0min Lifetime:2.6ns

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.


Usage and Biology

ssrA-LVA tag

In E. coli [wild-type (sspB+ clpX+)], the adaptor sspB binds ssrAtagged substrates to ClpXP proteases, resulting in a modest increase in their degradation rate.
LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

colorgreen
emission507
excitation488
tagssrA-LVA
The bacteria solution is green because of the expression of EGFP.
[edit]
Categories
Parameters
colorgreen
emission507
excitation488
tagssrA-LVA