Coding

Part:BBa_K4140016

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 09:56, 28 September 2022 by Ahmed A Rezk (Talk | contribs)


Cas12g


Part Description

RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 313
    Illegal PstI site found at 787
    Illegal PstI site found at 1729
    Illegal NgoMIV site found at 1230
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None