Characterizations of BBa_K4245130

Lambert iGEM collected data for basic parts BBa_K4245130 and BBa_K4245132 using the characterization protocol linked here. There is a negative logarithmic correlation between the complement concentrations ranging from 0.1-100 mM and the relative fluorescence units (RFU) (see Fig. 1). The 0 mM complement concentration produces less RFU than 0.1 mM, which does not align with the model. However, the large error bars at 0 mM suggests that there was some degree of significant error. Thus, this data point is insignificant and further trials should be performed to achieve more accurate results. Moreover, the data from 0.1-100 mM closely parallels the predictive ordinary differential equation (ODE) model (see Fig. 2) correlating miRNA to RFU (see Modeling). The complement concentration in the characterization curve mimics RCP, which is directly proportional to the initial miRNA concentration. Therefore, the overall data collected depicts an accurate relationship between the complement concentration and RFU.

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Figure 1. Characterization curve for parts BBa_K4245130 and BBa_K4245132 showing a negative logarithmic relationship between RFU and complement concentrations ranging from 0.1-100 μM. Note: 0-0.1 μM shows positive relationship, but large error bars at 0 μM suggest this was due to faulty pipetting.

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Figure 2. Deterministic ODE Model Simulation of RFU output dependent on concentration of miRNA.

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