Coding

Part:BBa_K4158012

Designed by: Satohiro Takizawa   Group: iGEM22_Waseda_Tokyo   (2022-09-23)
Revision as of 07:26, 25 September 2022 by S Okuda (Talk | contribs)


SRTF1(E coli Codon Optimized sequence)

RBS and coding site for the SRTF1　protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol((17β)-estra-1,3,5(10)-triene-3,17-diol) binds to edcR and inhibit its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal BamHI site found at 532
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
    Illegal AgeI site found at 173
    Illegal AgeI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None