Coding

Part:BBa_K4361314

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-19)
Revision as of 14:30, 22 September 2022 by Larsvdb (Talk | contribs)


BlcR A67V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.5 A67V (Part:BBa_K4361223). For this mutant, the alanine in position 67 has been changed to valine by mutating the GCG codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • G 482 > T, resulting in substitution G161V
  • G 670 > T, resulting in substitution A224S
  • G 677 > T and T 678 > G, resulting in substitution R226L
  • G 716 > C, resulting in substitution S239T
  • G 765 > A, silent mutation
  • G 787 > C, resulting in substitution A263P

Sequencing data only contained this part's sequence up until and including nucleotide 791. All nucleotides after are presumed to be identical to those found in the original codon optimized BlcR, Part:BBa_K4361100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589


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