Generator

Part:BBa_K237001:Design

Designed by: Marc T. K. Nielsen   Group: iGEM09_SDU-Denmark   (2009-08-03)
Revision as of 14:11, 3 August 2009 by Marc.mtk (Talk | contribs) (→‎Design Notes)

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IPTG inducible RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are 4 versions of RIP producers from us.

1 - Constitutively produced RIP without an export sequence

2 - Inducibly produced RIP without an export sequence

3 - Constitutively produced RIP with an export sequence (OmpA)

4 - Inducibly produced RIP with an export sequence (OmpA)

This is number 2

Source

The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus).

References