Regulatory
BLp

Part:BBa_K238013

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-08-02)
Revision as of 13:40, 2 August 2009 by AnneV (Talk | contribs)

Exact promoter of the blue light receptor

This is a promoter which normally lies before the ycgZ gene, a part of an operon consisting of several genes used by E.coli for regulating the structure of their biofilm. It is regualted by the ycgF/E system. This ssytem has a receptor, ycgF, which is responsive of blue light. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to the ycgE repressor through its EAL-domain releasing the repressor from the promoter region. the inverted repeats shown here is the DNA we expect ycgE to bind to when repressing the promoter. For more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aExact promoter of the blue light receptor