Part:BBa_K4160000
Igκ secretion signal
Igκ secretion signal is derived from the murine immunoglobulin kappa (IgGκ) light chain (Figure 1). This part is a well-characterized signal peptide for transgene expression1 and is widely implemented in several eukaryotic expression vectors.2 The Igκ secretion signal translocates proteins to the cellular membrane.3
Figure 1 | 3D structure of the IgGκ light chain. Yellow: the Igκ signal. Blue: main chain (AlphaFold: AF-P01661-F1).4
Usage and Biology
The Igκ secretion signal is derived from the IgGκ light chain that originates from the Mus musculus (mouse).4 This signal is a peptide that influences the targeting pathway of a protein, leading to specific post-translational modifications or translocation of proteins to the cellular membrane.1,3 Signal peptides are short peptides that consist of approximately 20 to 30 amino acids.1 It consists of a basic N-domain, a hydrophobic domain, and a slightly polar C-domain.3 Signal peptides are recognized during the translation of a secretory protein. The signal peptide will bind a signal recognition particle, to form a complex that halts the translation. Subsequently, this complex is transported to the endoplasmic reticulum. From there, the translation will resume and the protein will eventually be secreted via the cell's secretory pathway.3 In research, the Igκ secretion signal is often used to express recombinant proteins.2 The TU-Eindhoven 2022 used this part to translocate the GEMS receptor (BBa_K4160008) to the membrane of HEK293T cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| biology | Mus musculus (mouse) |