DNA

Part:BBa_K4361114

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-08)
Revision as of 09:00, 11 October 2022 by Larsvdb (Talk | contribs)


Blc operator sequence SDM deletion

This part shows the Blc operator sequence (Part:BBa_K4361111) after site-directed mutagenesis with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in the deletion of the adenine in position 20. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As such, when inserted in a reporter system such as Part:BBa_K4361115, it may act as a negative control against the wildtype operator sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None