Coding
Part:BBa_K4477002:Design
Designed by: Miranda Khoury Group: iGEM22_Virginia (2022-09-06)
Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - heavy chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 73
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1158
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amino acid sequence from IMGT was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.
The variable heavy and constant 1 domains of this heavy chain are human in origin, while the constant 2 and 3 domains are mouse in origin. See Team Virginia 2022's Benchling folder <a href="https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/">here</a> for detailed annotations.
Source
Orticumab heavy chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635