Composite

Part:BBa_K4390002

Designed by: Maarten van den Ancker, William McKenny   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-06)
Revision as of 16:26, 6 September 2022 by Will McX (Talk | contribs)


ArsR expression construct

BBa_K4390002 is a working transcriptional unit which expresses the ArsR repressor (BBa_J15101). It is composed of the constitutive promoter J23100 (BBa_J23100), a ribosome binding site (BBa_B0034), the arsR repressor sequence (BBa_J15101) and the weak synthetic L2U2H09 Terminator (BBa_K4390001). This unit was assembled using JUMP and can be assembled into a pJUMP29-1A KanR Type IIS Level 1 vector plasmid for expression in cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None