Coding

Part:BBa_K4390066

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-03)
Revision as of 15:47, 12 October 2022 by Dsam (Talk | contribs)


SpyCatcher O part

This is an O part consisting of two SpyCatcher domains separated by a glycine-serine linker. The SpyCatcher domains are used for binding and crosslinking proteins that have a SpyTag attached to them to create a mesh that can spontaneously form a hydrogel. The 2022 Edinburgh-UHAS iGEM team used an expressed level 1 construct containing this part to crosslink metallothioneins tagged with a SpyTag domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None